Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.9 extracted from

  • van Alebeek, G.; Tafazzul, G.; Kreuwels, M.; Keltjens, J.; Vogels, G.
    Cyclic 2,3-diphosphoglycerate metabolism in Methanobacterium thermoautotrophicum (strain DELTAH) characterization of the synthetase reaction (1994), Arch. Microbiol., 162, 193-198 .
No PubMed abstract available
Search for more literature for 6.5.1.9

Activating Compound

Activating Compound Comment Organism Structure
additional information presence of oxygen, dithiothreitol, or phosphate does not affect the reaction. Activation of the cDPG synthase is not required in contrast to the cDPG hydrolase Methanothermobacter thermautotrophicus

Inhibitors

Inhibitors Comment Organism Structure
additional information presence of oxygen, dithiothreitol, or phosphate does not affect the reaction Methanothermobacter thermautotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm upon ultracentrifugation of cell extract (135000 x g, 1 h, 4°C), the enzymatic activity is recovered exclusively in the supernatant, which indicates a cytoplasmic localization of the enzyme Methanothermobacter thermautotrophicus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 42% activity compared to Mg2+ Methanothermobacter thermautotrophicus
K+ activity strictly requires the presence of salt. Optimal activity is found at 0.5 M KC1, which can be replaced by 0.5 M K+-acetate (136%), but not by 0.5 M NaC1 (5.4%), indicating that the presence of the potassium ion is specifically needed for cDPG synthesis Methanothermobacter thermautotrophicus
Mg2+ Km = 3.1 mM from Lineweaver-Burk plot, best cation Methanothermobacter thermautotrophicus
Mn2+ 37% activity compared to Mg2+ Methanothermobacter thermautotrophicus
additional information Mg2+ can be partially replaced by Co2+ (42%), Mn2+ (37%), or Ni2+ (25%), but not by Ca2+, Zn2+, or Fe2+ Methanothermobacter thermautotrophicus
Ni2+ 25% activity compared to Mg2+ Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2,3-diphospho-D-glycerate Methanothermobacter thermautotrophicus
-
 ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ir
ATP + 2,3-diphospho-D-glycerate Methanothermobacter thermautotrophicus DSM 1053
-
 ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ir
ATP + 2,3-diphospho-D-glycerate Methanothermobacter thermautotrophicus DELTAH
-
 ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ir

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus DELTAH
-
-
-
Methanothermobacter thermautotrophicus DSM 1053
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 42fold by hydrophobic interaction chromatography, ultrafiltration, anion exchange chromatography, ultrafiltration, and dye affinity chromatography on a Procion Green HE-4BD resin, and again ultrafiltration Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0037
-
 crude enzyme extract, pH 6.0, 65°C Methanothermobacter thermautotrophicus
0.1548
-
 purified native enzyme, pH 6.0, 65°C Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2,3-diphospho-D-glycerate
-
 Methanothermobacter thermautotrophicus ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ir
ATP + 2,3-diphospho-D-glycerate
-
 Methanothermobacter thermautotrophicus DSM 1053 ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ir
ATP + 2,3-diphospho-D-glycerate
-
 Methanothermobacter thermautotrophicus DELTAH ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ir

Synonyms

Synonyms Comment Organism
cDPG synthase
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 cell extract, pH 6.0, l h, 50% activity reamining Methanothermobacter thermautotrophicus
65
-
 cell extract, pH 6.0, l h, 7% activity reamining Methanothermobacter thermautotrophicus
70
-
 cell extract, pH 6.0, l h, no activity reamining Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 narrow optimum Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanothermobacter thermautotrophicus

General Information

General Information Comment Organism
metabolism cDPG synthesis and hydrolysis in methanogenic bacteria are catalyzed by two different enzymes: cyclic 2,3-diphosphoglycerate (cDPG) synthetase and cDPG hydrolase. The hydrolase catalyzes the hydrolysis of cyclic 2,3-bisphosphoglycerate to 2,3-diphosphoglycerate. The reverse reaction is catalyzed by cDPG synthetase in an ATP-dependent manner. The routes of cDPG biosynthesis and degradation are interconnected Methanothermobacter thermautotrophicus